Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)

Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)

SKU #	PKSH033851
Expression Host	HEK293 Cells

Description

Synonyms	425O18-1,Angiopoietin-like protein 4,Angiopoietin-related protein 4,Angptl4,Farp,Fasting-induced adipose factor,Fiaf,HFARP,Hepatic fibrinogen/angiopoietin-related protein,Ng27,Secreted protein Bk89
Species	Human
Expression Host	HEK293 Cells
Sequence	Phe20-Thr263
Accession	Q6ZMC9
Calculated Molecular Weight	53.5 kDa
Observed Molecular Weight	50-80 kDa
Tag	C-Fc
Bio-activity	Loaded Human Siglec-15-Fc on AHC Biosensor, can bind Anti-Human Siglec15 mAb-mFc with an affinity constant of 0.13 nM as determined in BLI assay.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 m filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background
Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.